A variety of mutations in lentiviral vector expression systems have been shown to generate a non-integrating phenotype. We studied a novel 12 base-pair U3-long terminal repeats (LTR) integrase (IN) ...
To reduce the time dedicated to clone screening in the development of cell lines for stable virus production, a protocol merging the procedures of cell cloning and titer screening was implemented: the ...
While the traditional cloning workflow has been used in laboratories for decades, new techniques have emerged that allow for rapid assembly of multiple genetic fragments. The Electra Vector System, ...